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Kazuhisa Nishizawa, MD, PhD                       

1964       Born in Sapporo,   

1989       Graduated the University of Tokyo School of Medicine,

1989-1993  Graduate School of the University of Tokyo, Department of Neurobiology, The Institute for Brain Research

1993-1995  Research associate,  Department of Biophysics, Teikyo University

1995-1998  Post-doctoral fellow, Immunobiology, Dana-Farber Cancer Institute, Boston

1998-2003  Lecturer at Biochemistry, Teikyo University School of Medicine

2003-2005       Associate Professor of Biochemistry, Teikyo University School of Medicine

2006-       Professor of Clinical Laboratory Science, Teikyo University School of Medical Technology

2006       Chief, Clinical Laboratory Science, Teikyo University School of Medical Technology




Membership and editorial services


Dec 2017 onward --   Editor in Chief ___  Annals of Biomedical Research (ABR) __  http://escires.com/annals-of-biomedical-research


Feb 2016 - June 2018 --   Editor-in-Chief __ Biomedical Research and Clinical Practice (BRCP)


Mar 2017 onward --  Editor-in-Chief of J. Biophys. Chem.----_____http://www.scirp.org/journal/jbpc/


Feb, 2013 onward --  Editorial Board member of_ BioMed Research International__area of computational biology________http://www.hindawi.com/journals/bmri/editors/computational.biology/


2012 onward --  Editorial Board member of _______Dataset Papers in Physics (formerly Biophysics)


2010 onward -- Editorial Board member of Computational and Mathematical Methods in Medicine.----________http://www.hindawi.com/journals/cmmm/






Publication list


[ Computational studies on TM dimerization ]


Kazuhisa Nishizawa, Manami Nishizawa

Atomistic simulation analyses of sequence-nonspecific effects of lipid composition on transmembrane helical peptide dimerization 

Annals of Biomedical Research   2019, 2:114   (Open Access)


Manami Nishizawa and Kazuhisa Nishizawa

Force-field dependency of Leu-rich helical peptides dimerization energy in lipid bilayers. II. Limited transferability of united-atom simulation parameters to membrane-water interfaces

Annals of Biomedical Research   2018, 1: 113     (Open Access)


Manami Nishizawa and Kazuhisa Nishizawa

Force-field dependency of Leu-rich helical peptides dimerization energy in lipid bilayers. I. United-atom simulations show discrepancy from experiments and all-atom simulations

Annals of Biomedical Research   2018, 1: 112   (Open Access)


Manami Nishizawa and Kazuhisa Nishizawa

Sequence-nonspecific stabilization of transmembrane helical peptide dimer in lipid raft-like bilayers in atomistic simulations. II. Acyl chain order-associated changes in lipid-peptide contacts concordant with potential energy profiles

Annals of Biomedical Research  2018 1: 106@ Word format is:   http://gen.g.dgdg.jp/ABR-ms-1-106.pdf


Manami Nishizawa and Kazuhisa Nishizawa

Sequence-nonspecific stabilization of transmembrane helical peptide dimer in lipid raft-like bilayers in atomistic simulations. I. Dimerization free energy and impact of lipid-peptide potential energy.

Annals of Biomedical Research  2018, 1: 105@Word format is:   http://gen.g.dgdg.jp/ABR-ms-1-105.pdf


Manami Nishizawa and Kazuhisa Nishizawa

Cholesterol and saturated fatty acid stabilize dimerization of helical transmembrane peptides by lowering energy cost related to peptides desolvation from lipids upon dimerization: an insight from atomistic simulation

Biomedical Research and Clinical Practice  (2017)  2:1-8    doi: 10.15761/BRCP.1000131


Nishizawa M, Nishizawa K

Free energy of helical transmembrane peptide dimerization in OPLS-AA/Berger force field simulations: inaccuracy and implications for partner-specific Lennard-Jones parameters  between peptides and lipids

Molecular Simulation  (2016)  42: 916-926


Nishizawa, M, Nishizawa, K

Potential of mean force analysis of the self-association of leucine-rich transmembrane ƒŋ-helices: difference between atomistic and coarse-grained simulations.

J Chem Phys. (2014)  141 : 075101



[Other topics]


Kazuhisa Nishizawa, Reiko Seki

Epidemiological Studies of Uric Acid: A Mini-review with a Focus on Mendelian Randomization 

Annals of Biomedical Research 2020, 3(1): 119 


Reiko Seki, Kazuhisa Nishizawa

Neutrophil extracellular trap formation after sterile insults in lung, liver, heart and kidney: a mini-review 

Annals of Biomedical Research 2019, 2(1): 117 


A Kawanabe, M Hashimoto, M Nishizawa, K Nishizawa, H Narita, T Yonezawa, Y Jinno, S Sakata, A Nakagawa and Y Okamura.   The hydrophobic nature of a novel membrane interface regulates the enzyme activity of a voltage-sensing phosphatase

eLife  2018: e41653 


Kazuhisa Nishizawa and Manami Nishizawa

Extracellular heat shock proteins as a natural protector of cells and organisms: a review of recent studies

Annals of Biomedical Research 2018, 1:109@__ http://escires.com/articles/ABR-1-109.pdf


Reiko Seki and Kazuhisa Nishizawa

Inflammatory vs thrombotic states of platelets: a mini-review with a focus on functional dichotomy 

Annals of Biomedical Research  2018  1: 107   http://escires.com/articles/ABR-1-107.pdf


Manami Nishizawa and Kazuhisa Nishizawa

Pirfenidone, fluorofenidone and new antifibrotic drugs: biochemistry of direct and indirect effects. 

Annals of Biomedical Research      2018; 1:101 __  http://escires.com/articles/ABR-1-101.pdf


Manami Nishizawa and Kazuhisa Nishizawa

Commentary---Why have cholesterol and saturated fatty acids acquired proinflammatory roles during evolution? – A hypothesis from atomistic simulations showing sequence-nonspecific stabilization of peptide dimers in lipid raft-like bilayers

Biomedical Research and Clinical Practice  (2017)@ 2 (2):1-6  DOI: 10.15761/BRCP.1000137


Reiko Seki and Kazuhisa Nishizawa

Factors regulating Th17: a review

Biomedical Research and Clinical Practice (2016)  1 :4: 126-147  DOI:10.15761/BRCP.1000122@


Gnanasambandam R., C. Ghatak, A. Yasmann, K, Nishizawa, F. Sachs, A.S. Ladokhin, S. Sukharev, T.M. Suchyna@@

GsMTx4: Mechanism of Inhibiting Mechanosensitive Ion Channels

Biophys J__2017 ;112(1):31-45.


Kazuhisa Nishizawa and Reiko Seki

Mechanisms of immunosuppression by mesenchymal stromal cells: a review with a focus on molecules

Biomedical Research and Clinical Practice__(2016)  1(3):82-96; doi: 10.15761/BRCP.1000116


Nishizawa K

Low-grade endotoxemia, diet, and gut microbiota – an emphasis on the early events leading to dysfunction of the intestinal epithelial barrier -review article

Biomedical Research and Clinical Practice@ (2016)  1(2)  DOI: 10.15761/BRCP.1000110 


Nishizawa K

Biomedical research and clinical practice - open access - editorial for the inaugural issue

Biomedical Research and Clinical Practice  (2016)@ 1:  DOI: 10.15761/BRCP.1000101 



Kazuhisa Nishizawa; Manami Nishizawa; Radhakrishnan Gnanasambandam; Frederick Sachs; Sergei I Sukharev, Thomas M. Suchyna

Effects of Lys to Glu mutations in GsMTx4 on membrane binding, peptide orientation, and self-association propensity, as analyzed by molecular dynamics simulations

Biochimica et Biophysica Acta (BBA) - Biomembranes  (2015) 1848:_ 2767-2778



Nishizawa M, Nishizawa K

Molecular Dynamics Simulation Analysis of Membrane Defects and Pore Propensity of Hemifusion Diaphragms.

Biophys J  (2013)  104: 1038-1048,


Nishizawa M and Nishizawa K 

Coarse-grained simulations of branched bilayer membranes: effects of

cholesterol-dependent phase separation on curvature-driven lipid sorting  

J Biophys Chem (2011)  2 : 268-284    DOI: 10.4236/jbpc.2011.23032  


Nishizawa M and Nishizawa K  

Curvature-driven lipid sorting: coarse-grained dynamics simulations of a membrane mimicking a hemifusion intermediate

J Biophys Chem (2010)   1:86-95


Nishizawa M, Nishizawa K

Molecular dynamics simulation analyses of viral fusion peptides in membranes prone to phase transition:

effects on membrane curvature, phase behavior and lipid-water interface destabilization.

J Biophys Chem (2010)   1:19-32


Nishizawa M, Nishizawa K

Coupling of S4 helix Translocation and S6 Gating Analyzed by Molecular Dynamic Simulations of Mutated Kv channels

Biophys J  (2009)    97: 90-100


Nishizawa M, Nishizawa K

Molecular Dynamics Simulation of Kv channel Voltage Sensor Helix in a Lipid Membrane with Applied Electric Field.

Biophys J  (2008)@ 95:1729-1744


Nishizawa M, Nishizawa K.

Molecular Dynamics Simulations of a Stretch-Activated Channel Inhibitor GsMTx4 with Lipid Membranes: Two Binding Modes and Effects of Lipid Structure.

Biophys J  (2007)   92: 4233-4243


Nishizawa M, Nishizawa K

Interaction between K+ Channel Gate Modifier Hanatoxin and Lipid Bilayer Membranes Analyzed by Molecular Dynamics Simulation.

Eur Biophys J  (2006)  35: 373-381


Nishizawa M, Nishizawa K.

 On slippage-like mutation dynamics within genes: a study of pseudogenes and 3'UTRs.

J Mol Evol. (2005) 60:274-275.



M Nishizawa and K Nishizawa

A DNA sequence evolution analysis generalized by simulation and the Markov chain Monte Carlo method implicates strand slippage in a majority of insertions and deletions.
J Mol Evol.   55: 706-717.(2002)


Wada Y, Yamashita T, Imai K, Miura R, Takao K, Nishi M, Takeshima H, Asano T, Morishita R, Nishizawa K, Kokubun S, Nukada T.

 A region of the sulfonylurea receptor critical for a modulation of ATP-sensitive K(+) channels by G-protein betagamma-subunits.

EMBO J. i2000j@19: 4915-4925. 


M. Nishizawa and K. Nishizawa

Amino acid and nucleotide recurrence in aligned sequences: synonymous substitution patterns in association with global and local base compositions

Nucleic Acids Res.  28: 3801-3810. (2000)


K. Nishizawa, M. Nishizawa and Ki Seok Kim

Tendency for local repetitiveness in amino acid usages in modern human proteins

J Mol Biol 294, 937-953 (1999)


M. Nishizawa and K. Nishizawa

Local repetitiveness in amino acids usages in protein sequences - a genomic factor for protein evolution

Proteins 37, 284-292 (1999)


Freund C, Dotsch V, Nishizawa K, Reinherz EL, Wagner G

The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences.

Nat Struct Biol 6:656-60 (1999)


Li J, Nishizawa K, An W, Hussey RE, Lialios FE, Salgia R, Sunder-Plassmann R, Reinherz EL

A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion.

EMBO J 17,7320-7336 (1998)


Kazuhisa Nishizawa, Christian Freund, Jing Li, Gerhard Wagner and Ellis L.Reinherz.

Identification of a novel proline-binding motif regulating CD2-triggered T lymphocyte activation.

Proc Natl Acad Sci USA 95,14897-902.(1998)


M. Nishizawa and K. Nishizawa

Biased usages of arginines and lysines in proteins are correlated with local-scale fluctuations of G+C content of DNA sequences.

J Mol Evol, 47, 385-93. (1998)


Kazuhisa Nishizawa and Shigeo Koyasu

IL-2 and IL-7 differentially induce CD4-CD8- ab TCR NK1.1+ large granular lymphocytes and IL-4 producing T cells from CD4-CD8- ab TCR+ NK1.1- cells:implications for the regulations of Th1 and Th2 type responces.

International Immunology, 9, 1123-1129 (1997)


Nishizawa K, Okado H and Okamoto H

An immunocytochemical technique for analysis of regulation of genes encoding early differentiation marker antigens in an oocyte translation system.

Biotech Histochem. 71, 73-78. (1996)


Nishizawa K, Shimoda E and Kasahara M

Substrate recognition domain of the Gal2 galactose transporter in yeast Saccharomyces cerevisiae as revealed by chimeric galactose-glucose transporters.

J Biol Chem. 270, 2423-2426. (1995)


Nishizawa K and Okamoto H

Mutation analysis of the role for the carboxy-terminus encoding region in NGF-induced stabilization of GAP-43 mRNA.

Biochem Biophys Res Commun. 205, 1380-1385. (1994)


Nishizawa K.

NGF-induced stabilization of GAP-43 mRNA is mediated by both 3' untranslated region and a segment encoding the carboxy-terminus peptide.

Biochem Biophys Res Commun. 200, 789-796. (1994)



iReview articles of earlier publicationj

Nishizawa K.

Control of stability of eukaryote mRNA--3' untranslated region as a program for degradation

Tanpakushitsu Kakusan Koso. (1995) 40, 1092-1099.


Nishizawa K.

Translation and Control of stability of mRNAs

in New development of Molecular Biology. M. Muramatsu ed. (1998) Asakura Press, Tokyo, Japanese.


Nishizawa K.

Gene control, Translation etc

Dictionary of Molecular and Cellular Biology. (1997) Tokyo-Kagaku-Dojin,

Tokyo, Japanese.


Nishizawa K.

Local repetitiveness and slippage-like mutations in genomic sequences.

Recent Research Developments in Molecular Biology  1,145-164 (2003) ,



Nishizawa K

Molecular Dynamics Simulations of Kv Channels and Gating-Modifier Peptide Toxins

Current Computer-Aided Drug Design  (2009) 5:155-173


Nishizawa K. Atomistic molecular simulation of gating modifier venom peptides: two binding modes and effects of lipid structure.

 in Mechanosensitivity of Cells and Tissues: Mechanosensititvity and Mechanotransduction by Andre Kamkin ed @(2010)







Teaching experiences

2006- Biochemistry, Clinical Chemistry, Analytical Chemistry, Special course of Clinical laboratory science I and II, Training course of Biochemistry,

Prior to 2006 when Dept of Clinical Lab Science was launched, I had class on Biochemistry, Medical English, and Cellular Biomedicine in Teikyo Univ School of Med.




Education of postgraduates (Master and Doctoral courses)

-- 2016, currently teaching one post-graduate toward her doctoral thesis work

-- 2013-  Classes on molecular metabolisms and clinical chemistry.